61516
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- Chinese name: Alkaline phosphatase
- English name: Alkaline Phosphatase
The enzyme is widely used as a marker in ALP-based immunoassay.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | clear and almost colorless liquid |
| Activity | ≧100,000 DEA U/mL |
| Specific activity | ≧6,000 DEA U/mg protein |
| Protein | 10–20 mg protein/mL, Biuret |
| Stabilizer | glycerol |
| Storage condition | at 2∼7℃ (do not freeze) |
Characteristics
| Molecular weight | ca. 140 kDa (gel filtration) |
| pH Optimum | 9.5–10.0 |
| pH Stability | 6.5–11.0 |
| Optimum temperature | 50℃ |
| Thermal stability | below ca. 40℃ |
| Stability (liquid form) | stable at 37℃ for at least four weeks |
| Stability (liquid form) | stable at 5℃ for at least two years |
| Activators | Mg 2+ 'Tris buffer, diethanolamine' |
| Inhibitors | inorganic phosphate, ethylenediaminetetraacetic acid (EDTA) |
Applications
The enzyme is widely used as a marker in ALP based immunoassay.
References
O’Sullivan, M. J. et al., Anal. Biochem., 100, 100–108 (1979).
Bergmeyer, H. U., “Methods of Enzymatic Analysis,” Vol. 2 (3rd ed.), Verlag Chemie, Weinheim, Germany, 1983, pp. 269–270.
Crowther, J. R., “ELISA: Theory and Practice,” Humana Press, New York, 1995.
Price, C. P. and Newman, D. J., “Principles and Practice of Immunoassay,” Macmillan Press, London, 1997.
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