61222
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- Chinese name: Glutamine synthetase
- English name: Glutamine Synthetase
This enzyme is useful for determining ammonia and ATP in clinical analysis.
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- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | white to light yellow lyophilizate |
| Activity | ≧15.0 U/mg |
| Contaminants | Catalase ≦0.5 U/U％ |
| Stabilizer | sucrose |
| Storage condition | below -20℃ |
Characteristics
| Molecular weight | ca. 900 kDa (gel filtration) |
| Structure | 57 kDa (SDS-PAGE) |
| Isoelectric point | 6.5 |
| Michaelis constant | 1.5×10 -2 M (L-glutamate) 1.3×10 -4 M (ammonia) 8.7×10 -4 M (ATP) |
| pH Optimum | ca. 7.0 |
| pH Stability | 6.5–9.5 |
| Optimum temperature | 60℃ |
| Thermal stability | below 40℃ |
| Inhibitors | methionine sulfoximine, carbamyl phosphate |
| Activators | Mg 2+ , Mn 2+ |
| Specificity | L-glutamate (100), D-glutamate (0.8) NH₃(100), NH₂OH (12) ATP (100), GTP (2.5) |
Applications
The enzyme is useful for the determination of ammonia and ATP in clinical analysis.
References
Ebner, E. et al., “Methods in Enzymology,” Vol. 17A, Academic Press, New York, 1970, pp. 910–922.
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