61170
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- Chinese name: Lactate dehydrogenase
- English name: Lactate Dehydrogenase
This enzyme is useful for determining numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate, and glycerol) and enzyme activities (GPT, PK, and CPK) when coupled with the relevant enzymes.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | white lyophilizate |
| Activity | ≧150 U/mg |
| Storage condition | below -20℃ |
Characteristics
| Molecular weight | ca. 115 kDa (gel filtration) |
| Michaelis constant | 2.5×10½M (lactate) 1.0×10¼M (pyruvate) |
| pH Optimum | 7.4–8.0 |
| pH Stability | 7.0–7.5 |
| Optimum temperature | 50–55℃ |
| Thermal stability | below 45℃ |
Applications
The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.
References
Jaenicke, R. and Pfleiderer, G., Biochim. Biophys. Acta, 60, 615 (1962).
Everse, J. and Kaplan, N. O., Adv. in Enzymol., 37, 61 (1973).
Holbrook, J. J. et al., “The Enzyme,” Vol. 11 (3rd ed.), Academic Press, New York and London, 1975, pp. 191–292.
Loshon, C. A. et al., Clin.Chem., 23, 1579 (1977).
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