61156
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- Chinese name: Urease
- English name: Uricase
This enzyme is useful for determining uric acid in clinical analysis.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | light brownish lyophilizate |
| Activity | ≧4.0 U/mg |
| Contaminants | Catalase ≦1.0 U/U% |
| Stabilizer | sucrose, citrate, ethylenediaminetetraacetic acid (EDTA) |
| Storage condition | below -20℃ |
Characteristics
| Molecular weight | ca. 90 kDa (gel filtration) |
| Structure | 2 subunits of 35 kDa (SDS-PAGE) |
| Michaelis constant | 1.1×10 -5 M (uric acid) |
| pH Optimum | 8.5 |
| pH Stability | 7.0–11.0 |
| Optimum temperature | 45℃ |
| Thermal stability | below 55℃ |
| Stability (liquid form) | stable at 37℃ for at least ten days |
| Stability (powder form) | stable at 30℃ for at least three weeks |
| Inhibitors | Hg 2+ , Ag + |
Applications
The enzyme is useful for the determination of uric acid in clinical analysis.
References
Koyama, Y. et al., J. Biochem., 120, 969–973 (1996).
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