60273
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- Chinese name: Fructosyl-amino acid oxidase
- English name: Fructosyl-amino Acid Oxidase
This enzyme is useful for determining fructosyl-L-amino acid.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | yellow lyophilizate |
| Activity | ≧4.0 U/mg |
| Stabilizer | trehalose |
| Storage condition | below -20℃ protected from light |
Characteristics
| Molecular weight | ca. 45 kDa (gel filtration) |
| Structure | monomer of 50 kDa (SDS-PAGE) |
| Michaelis constant | 2.2×10 -4 M (N ε -fructosyl-L-lysine) |
| pH Optimum | 8.0–8.5 |
| pH Stability | 6.0–8.5 |
| Optimum temperature | 35–40℃ |
| Thermal stability | below 30℃ |
| Stability (liquid form) | stable at 25℃ for at least two weeks |
| Stability (powder form) | stable at 37℃ for at least three weeks |
| Inhibitors | Ag + ，Cu 2+ |
| Specificity | N ε -fructosyl-L-lysine (100), fructosyl-L-valine (65) |
| fructosyl-glycine (30) |
Applications
The enzyme is useful for the determination of fructosyl-L-amino acid.
References
Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).
Sakaue, R. et al., Biosci. Biotechnol. Biochem., 66, 1256–1261 (2002).
Hirokawa, K. and Kajiyama, N., Biosci. Biotechnol. Biochem., 66, 2323–2329 (2002).
Sakaue, R. and Kajiyama, N., Appl. Environ. Microbiol., 69, 139–145 (2003).
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