60266
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- Chinese name: Cholesterol oxidase
- English name: Cholesterol Oxidase
This enzyme is useful for determining cholesterol in clinical analysis.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | yellow liquid | |
| Activity | ≧200 U/mL | |
| Contaminants | Catalase ≦2.0 U/U% | |
| pH | 7.3–7.7 | |
| Stabilizer | sucrose | |
| Storage condition | below -20℃ protected from light |
Characteristics
| Molecular weight | ca. 59 kDa (gel filtration) |
| Structure | monomer of 60 kDa (SDS-PAGE) |
| Michaelis constant | 1.9×10 -5 M (cholesterol) |
| pH Optimum | 6.5–8.0 |
| pH Stability | 3.5–8.5 |
| Optimum temperature | 55℃–65℃ |
| Thermal stability | below 70℃ |
| Stability (liquid form) | stable at 25℃ for at least one month |
| Inhibitors | Ag + , Hg 2+ |
Applications
The enzyme is useful for the determination of cholesterol in clinical analysis.
References
Aono, R. et al., Appl. Environ. Microbiol., 60, 2518–2523 (1994).
Doukyu, N. et al., Appl. Environ. Microbiol., 64, 1929–1932 (1998).
Doukyu, N. and Aono, R., Biochem. J., 341, 621–627 (1999).
Doukyu, N. and Aono, R., Appl. Microbiol. Biotechnol., 57, 146–152 (2001).
Aunpad, R. et al., Acta Cryst., D58, 2182–2183 (2002).
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