60241
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- Chinese name: Glutamate dehydrogenase
- English name: Alpha-Glucosidase
This enzyme is useful for determining α-amylase and serum chloride in clinical analysis.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | white lyophilizate | |
| Activity | ≧10 U/mg | |
| Stabilizer | bovine serum albumin | |
| Storage condition | below -20℃ |
Characteristics
| Molecular weight | ca. 61 kDa (gel filtration) |
| Structure | monomer of 60 kDa (SDS-PAGE) |
| Michaelis constant | 5.5×10 -5 M (p-nitrophenyl-α-D-glucoside) |
| pH Optimum | 6.0–9.0 |
| pH Stability | 5.0–10.0 |
| Optimum temperature | 52–55°C |
| Thermal stability | below 50℃ |
| Stability (liquid form) | stable at 37℃ for at least two weeks |
| Stability (powder form) | stable at 30℃ for at least one month |
| Inhibitors | Hg 2+ ，Ag + |
| Specificity | Refer to the table shown below. |
Applications
The enzyme is useful for the determination of α-amylase and serum chloride in clinical analysis.
References
Halvorson, H., “Methods in Enzymology,” Vol. 8, Academic Press, New York, 1966, pp. 559–562.
Ono, T. et al., Clin. Chem., 34, 552–553 (1988).
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