60233
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- Chinese name: Maltose phosphorylase
- English name: Maltose Phosphorylase
This enzyme is useful for determining α-amylase and inorganic phosphate in clinical analysis.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | white lyophilizate | |
| Activity | ≧10 U/mg | |
| Stabilizer | lactose, ethylenediaminetetraacetic acid (EDTA) | |
| Storage condition | below -20℃ |
Characteristics
| Molecular weight |
ca. 220 kDa (gel filtration) |
| Structure | 2 subunits of 90 kDa (SDS-PAGE) |
| Michaelis constant |
1.9×10 -3 M (maltose) |
|
3.4×10 -3 M (phosphate) |
|
|
8.3×10 -3 M (arsenate) |
|
| pH Optimum | 6.5–7.5 |
| pH Stability | 5.5–8.0 |
| Optimum temperature | 45–50°C |
| Thermal stability | below 55℃ |
| Stability (liquid form) | stable at 37℃ for at least one week |
| Stability (powder form) | stable at 30℃ for at least four weeks |
| Inhibitors |
Hg 2+ ，Ag + ，Zn 2+ ，Cu 2+ |
Applications
The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.
References
Hiruma, M. et al., Nippon Nogeikagaku Kaishi, 70, 773–780 (1996).
Shirokane, Y. et al., Carbohydr. Res., 329, 699–702 (2000).
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