60123
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- Chinese name: Fructosyl-amino acid oxidase CE
- English name: Fructosyl-peptide Oxidase (FPOX-CE)
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | yellow lyophilizate |
| Activity |
≧6.0 U/mg |
| Contaminants | Catalase ≦1.0 U/U％ |
| Stabilizer | ethylenediaminetetraacetic acid (EDTA), sodium glutamate |
| Storage condition | below -20℃ protected from light |
Characteristics
| Molecular weight | ca. 60kDa (gel filtration) |
| Structure | monomer of 52 kDa (SDS-PAGE) |
| Isoelectric point | 4.2 |
| Michaelis constant | 3.4×10 -3 M (fructosyl-valyl-histidine) |
| 4.4×10 -3 M (fructosyl-glycine) | |
| 8.9×10 -3 M (N ε -fructosyl-lysine) | |
| pH Optimum | 7.5–8.0 |
| pH Stability | 6.0–9.5 |
| Optimum temperature | 35–42℃ |
| Thermal stability | below 45℃ |
| Stability (powder form) | stable at 37℃ for at least one month |
| Specificity | fructosyl-valyl-histidine (100), fructosyl-glycine (53) |
| N ε -fructosyl-lysine (84) |
Application
The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.
References
Hirokawa K, Gomi K, Bakke M, Kajiyama N. (2003)
Distribution and properties of novel deglycating enzymes for fructosyl peptide in fungi.
Archives of Microbiology, 180, 227-231.
Hirokawa K, Gomi K, Kajiyama N. (2003)
Molecular cloning and expression of novel fructosyl peptide oxidases and their application for the measurement of glycated protein.
Biochem Biophys Res Commun, 311(1), 104-111.
Hirokawa K, Shimoji K, Kajiyama N. (2005)
An enzymatic method for the determination of hemoglobinA1C.
Biotechnology Letters, 27(14), 963-968.
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