60122
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- Chinese name: Sucrose phosphorylase
- English name: Sucrose Phosphorylase
This enzyme is useful for determining inorganic phosphate in clinical analysis.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | white lyophilizate |
| Activity | ≧50 U/mg |
| Stabilizer | sucrose |
| Storage condition | below -20℃ |
Characteristics
| Molecular weight | ca. 56 kDa (gel filtration) |
| Structure | monomer of 56 kDa (SDS-PAGE) |
| Isoelectric point | 4.6 |
| Michaelis constant | 3.9×10 -2 M (sucrose) |
| 6.2×10 -3 M (phosphate) | |
| pH Optimum | 7.5 |
| pH Stability | 5.0–8.0 |
| Optimum temperature | 40℃ |
| Thermal stability | below 45℃ |
| Stability (liquid form) | stable at 37℃ for at least two weeks |
| Stability (powder form) | stable at 30℃ for at least two weeks |
| Inhibitors | glucose, glucono-1,5-lactone |
| Specificity | sucrose (100), maltose (0), starch (0) |
Applications
The enzyme is useful for the determination of inorganic phosphate in clinical analysis.
References
Koga, T. et al., Agric. Biol. Chem., 55, 1805–1810 (1991).
Kitao, S. and Nakano, E., J. Ferment. Bioeng., 73, 179–184 (1992).
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