60111
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- Chinese name: Creatinine enzyme
- English name: Creatininase (C1-E)
The enzyme is useful for determining creatinine in clinical analysis.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | white lyophilizate | |
| Activity | 600-750 U/mg | |
| Contaminants | Catalase ≤0.5% | |
| Stabilizer | sucrose | |
| Storage condition | below -20℃ |
Characteristics
| Molecular weight | ca. 170 kDa (gel filtration) |
| Structure | 6 subunits of 28 kDa (SDS-PAGE) |
| Isoelectric point | 4.8 |
| Michaelis constant | 3.4×10 -2 M (creatinine) |
| 4.3×10 -2 M (creatine) | |
| pH Optimum | 6.5–7.0 |
| pH Stability | 7.0–11.0 |
| Optimum temperature | 60–65℃ |
| Thermal stability | below 60℃ |
| Stability (liquid form) | stable at 37°C for at least two weeks |
| Stability (powder form) | stable at 30°C for at least one month |
| Inhibitors | Hg 2+ |
| Activators | Mg 2+ ，Mn 2+ |
Applications
The enzyme is useful for the determination of creatinine in clinical analysis.
References
Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.
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