60105
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- Chinese name: Sarcosine oxidase
- English name: Sarcosine Oxidase
This enzyme is useful for determining creatinine and creatine in clinical analysis.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | yellow lyophilizate |
| Activity | ">=20 U/mg |
| Contaminants | Creatininase <1.0 x 10-2 U/U% -2 U/U% |
| Stabilizer | sucrose |
| Storage condition | below -20℃ protected from light |
Characteristics
| Molecular weight | ca. 49 kDa (gel filtration) |
| Structure | monomer of 43 kDa (SDS-PAGE) |
| one mole of FAD per mole of enzyme | |
| Isoelectric point | 5.3 |
| Michaelis constant | 4.7 x 10-3 M (sarcosine) -3 M (sarcosine) |
| pH Optimum | 6.7–9.5 |
| pH Stability | 6.5–10.5 |
| Optimum temperature | 50℃ |
| Thermal stability | below 55℃ |
| Stability (liquid form) | stable at 37℃ for at least two weeks |
| Stability (powder form) | stable at 30℃ for at least one month |
| Inhibitors | Zn2+, Cu, Hg, Ag 2+ , Cu 2+ , Hg 2+ , Ag + |
Applications
The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
References
Suzuki, M. and Yoshida, M. (1984)
A new enzymatic determination of serum creatine
Clinica Chimica Acta, 140, 289–294.
Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.
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