60101
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- Chinese name: Glucose dehydrogenase
- English name: Glucose Dehydrogenase
This enzyme is useful for determining D-glucose in clinical analysis and self-monitoring blood glucose meters.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | yellow lyophilizate |
| Activity | ">=700 U/mg |
| Contaminants | NAD glucose dehydrogenase <1.0 x 10 -2 U/U% |
| Hexokinase <1.0 x 10 -2 U/U% | |
| α-glucosidase <1.0 x 10 -2 U/U% | |
| β-glucosidase <1.0 x 10 -2 U/U% | |
| Filterability | ">=90% |
| Storage condition | below -20℃ protected from light |
Characteristics
| Molecular weight | ca. 85 kDa (SDS-PAGE) |
| Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
| Michaelis constant | 2.2 x 10 -2 M (D-glucose) |
| pH Optimum | 7.0–7.5 |
| pH Stability | 4.5–8.0 |
| Optimum temperature | 40–50℃ |
| Thermal stability | below 45℃ |
| Specificity | D-glucose (100), maltose (<0.01), |
| D-xylose (8.7), D-galactose (0.7), | |
sucrose (0.1), D-mannnose (3.9), 2-deoxy-d-glucose (52.2) |
Applications
The enzyme is useful for the determination of D-glucose in clinical analysis and self-monitoring blood glucose meters.
References
Hatada M, Loew N, Inose-Takahashi Y, Okuda-Shimazaki J, Tsugawa W, Mulchandani A, Sode K (2018)
Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference.
Bioelectrochemistry, 121, 185-190
Okuda-Shimazaki, J, Yoshida H, Sode K (2020)
FAD dependent glucose dehydrogenases – Discovery and engineering of representative glucose sensing enzymes –
Bioelectrochemistry, 132, 107414
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