60118
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- Chinese name: Creatine kinase AT
- English name: Creatinase (C2-AT)
This enzyme is useful for determining creatinine and creatine in clinical analysis.
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- Application: Biodiagnostic
- Product category: Enzyme
- Biochemical diagnosis: Colorimetric method
Specifications
| Appearance | white to light yellow lyophilizate |
| Activity | ≧9 U/mg |
| Stabilizer | sucrose |
| Storage condition | below -20℃ |
Characteristics
| Molecular weight | ca. 80 kDa (gel filtration) |
| Structure | 2 subunits of 48 kDa (SDS-PAGE) |
| Michaelis constant | 8.6×10 -3 M (creatine) |
| pH Optimum | 7.0–9.0 |
| pH Stability | 4.0–11.0 |
| Optimum temperature | 45℃ |
| Thermal stability | below 53℃ |
| Stability (liquid form) | stable at 37℃ for at least two weeks |
| Stability (powder form) | stable at 30℃ for at least one month |
| Inhibitors | Hg 2+ |
Applications
The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
References
Suzuki, M., Medical Technology, 7, 945–950 (1979).
Suzuki, M. and Yoshida, M. (1984)
A new enzymatic determination of serum creatine
Clinica Chimica Acta, 140, 289–294.
Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.
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